Nitrosyl hemochrome in pork products was measured using the method described by
Hornsey (1956) (link). After extraction and
filtration, absorbance of the filtrate was determined at 540 nm
(A540) using a spectrophotometer (UV-1800, Shimadzu, Kyoto, Japan).
Nitrosyl hemochrome concentration (ppm) was calculated by multiplying absorbance
(A540) by 290. TBARS values was measured using the method
described by Tarladgis et al. (1960) (link).
Briefly, after reacting malondialdehyde (MDA) in samples with 0.02 M
2-thiobarbituric acid (TBA) solution, absorbance of reactive substances was
determined at 538 nm. The results were multiplied by a factor of 7.8 to
calculate TBARS values (mg MDA/kg samples).
Hornsey (1956) (link). After extraction and
filtration, absorbance of the filtrate was determined at 540 nm
(A540) using a spectrophotometer (UV-1800, Shimadzu, Kyoto, Japan).
Nitrosyl hemochrome concentration (ppm) was calculated by multiplying absorbance
(A540) by 290. TBARS values was measured using the method
described by Tarladgis et al. (1960) (link).
Briefly, after reacting malondialdehyde (MDA) in samples with 0.02 M
2-thiobarbituric acid (TBA) solution, absorbance of reactive substances was
determined at 538 nm. The results were multiplied by a factor of 7.8 to
calculate TBARS values (mg MDA/kg samples).
