Quantifying NHEJ and SSA DNA Repair
Corresponding Organization :
Other organizations : University of Alabama at Birmingham
Variable analysis
- Plasmid type (pSF-tdTomato-END for NHEJ repair and pSF-tdTomato-HOM for SSA repair)
- Repair efficiency (proportion of EYFP+ cells among viable, tdTomato+ transfected cells)
- Cell types investigated
- Plasmid amount (400ng)
- Transfection method (P3 Primary cells nucleofection kit on 4D Nucleofector system)
- Electroporation format (20μl, strips of electrocuvettes, EO-115 program)
- Recovery time (12h)
- Staining with DAPI to identify viable cells
- Not explicitly mentioned
- Not explicitly mentioned
Annotations
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