Cultivation of KSHV-infected Cell Lines

293T cells (kind gift of Lori Frappier, University of Toronto) were grown at 37°C in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and glutamine. iSLK cells [58 (link)] (gift of Don Ganem, UCSF) were maintained in DMEM containing 10% charcoal stripped FBS (Sigma) and 1% glutamine with 250μg/ml G-418 and 1μg/ml puromycin. iSLK cells were infected with WT KSHV derived from bacmid BAC16, expressing eGFP and hygromycin resistance [28 (link)]. Bac16 KSHV infected iSLK cells were maintained in 1.2 mg/ml hygromycin, 250μg/ml G-418 and 1μg/ml puromycin. TRExBCBL1-Rta (kindly provided by Prof. J. Jung) were cultured in RPMI 1640 10% Tet System approved FBS (Clontech) and 1% glutamine with 50μg/ml hygromycin.