Antibody Identification and Protein Analysis in Synucleinopathies

Primary antibodies used are as follows: α-synuclein monoclonal antibodies Ab274 and Ab62 from our laboratory,^{51 (link)}α-synuclein, AMPK, p-AMPK, and p62 antibodies (Cell Signaling Technology, Danvers, MA, USA), p62 (BD Biosciences, San Jose, CA, USA), secretogranin II (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), LC3B antibody (Abcam, Cambridge, UK), and β-actin antibody (Sigma-Aldrich Corp., St. Louis, MO, USA). A monoclonal antibody for LAMP2 (H4B4) developed by Drs August and Hildreth was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD, National Institutes of Health and maintained by the University of Iowa, Department of Biological Sciences, Iowa City, IA, USA.
Retinoic acid, poly-L-lysine, trehalose, sorbitol, sucrose, maltose, rapamycin, CQ, trehalase, invertase, and acarbose were also purchased from Sigma. BafA1 was purchased from EMD Millipore (Billerica, MA, USA). pLVX-mCherry-Gal3 plasmid was a kind gift from Dr. Edward M Campbell.^{52 (link)}