Investigating SEMA3C Regulation in Cells

Anti-ACTIN, Anti-ULK1, Anti-p-ULK1, Anti-p62, and Anti-LC3B were purchased from Cell Signaling Technology (Danvers, MA). The SEMA3C and GAPDH antibodies were purchased from Proteintech (Rosemont, IL). The Anti-KRAS antibody was from ABclonal (Woburn, MA). CellTiter-Glo^®2.0 was purchased from Sigma-Aldrich (St. Louis, MO). ECL Chemiluminescence kit was obtained from National Diagnostics (Atlanta, Georgia). Recombinant Human SEMA3C protein was purchased from the R&D Systems (Minneapolis, MN). EasySep™ Mouse CD8+ T Cell Isolation Kit was from Stem Cell (Cambridge, MA). Human KRAS (G12D) Expression Lentivirus was from GenTarget Inc (San Diego, CA). SEMA3C plasmids were purchased from Sino Biological (Wayne, PA). The SEMA3C inhibitor, 3,5,4’-Tribromosalicylanilide (CAS: 87-10-5), was purchased from TCI chemicals (Portland, OR) (26 (link)).