Bacterial Identification by 16S rRNA Sequencing

Identification of strain S14E4C was done based on 16S rRNA gene sequencing: the genomic DNA of strain S14E4C was extracted using a DNA extraction kit (DNeasy Power Lyzer Microbial Kit, Qiagen, Germany). To confirm the identities of the isolates the 16S rRNA gene was amplified (PCR) from the extracted genomic DNA using the universal primers 27f (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492r (5′-GGCTACCTTGTT ACGACTT-3′) [5 (link)] (LGC Genomics, Berlin, Germany). The 16S rRNA gene sequence of strain S14E4C was compared with references in the EzTaxon database [6 (link)] and the NCBI Nucleotide database using BLAST [7 (link)] to identify closely related bacteria.