De novo sequencing and annotation of DijuNPV

DijuNPV genomic DNA was sequenced with two different high-throughput sequencing approaches: the 454 Genome Sequencer (GS) FLX™ Standard (Roche) at the ‘Centro de Genômica de Alto Desempenho do Distrito Federal’ (Center of High-Performance Genomic, Brasilia, Brazil) and the Illumina HiSeq™ 2000 platform at Macrogen Inc. (Seoul, Republic of Korea). The 454 sequencing data was assembled de novo using Geneious 9.0 [21 (link)], and the in silico-predicted restriction enzyme digestion profile was compared to Ribeiro et al. [13 ]. The open reading frames (ORFs) that started with a methionine codon (ATG) and encoded polypeptides of at least 50 amino acids were identified with Geneious 9.0 and annotated using BLASTX [22 (link)]. An acceptable overlap of less than 50% of the ORF within the neighbor ORFs was considered using a more liberal ORF annotation criterion based on Ref. [23 (link)]. Tandem Repeats Finder (http://tandem.bu.edu/trf/trf.html) [24 (link)] implemented in Geneious 9.0 was used to locate repeat regions. The genomic DNA sequence was submitted to the GenBank with the accession number MK558262.