Laser Microdissection of Arcuate Nucleus

Coronal cryostat sections (80 μm) of the hypothalamic arcuate nucleus (ARC) were placed on PEN-coated slides (Leica Microsystems, Wetzlar, Germany) (Tsukita et al., 2012 (link)). Laser microdissection was carried out on a Leica AS LMD (Leica Microsystems, Wetzlar, Germany) (Asai et al., 2017 (link)). Immediately after microdissection, total RNA was purified using an RNeasy Micro Kit (Qiagen, Valencia, CA, USA) (Tsukita et al., 2017 (link)).

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Hosaka S., Yamada T., Takahashi K., Dan T., Kaneko K., Kodama S., Asai Y., Munakata Y., Endo A., Sugawara H., Kawana Y., Yamamoto J., Izumi T., Sawada S., Imai J., Miyata T, & Katagiri H. (2020). Inhibition of Plasminogen Activator Inhibitor-1 Activation Suppresses High Fat Diet-Induced Weight Gain via Alleviation of Hypothalamic Leptin Resistance. Frontiers in Pharmacology, 11, 943.

Publication 2020

PEN-coated slides Leica AS LMD RNeasy Micro Kit

Hypothalamic arcuate nucleus Microdissection

Corresponding Organization : Tohoku University

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Variable analysis

independent variables

Laser microdissection carried out on a Leica AS LMD

dependent variables

Total RNA purified using an RNeasy Micro Kit

control variables

Coronal cryostat sections (80 μm) of the hypothalamic arcuate nucleus (ARC)
Slides coated with PEN (Leica Microsystems, Wetzlar, Germany)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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