Chlorophyll contents in leaves from 2‐wk‐old wild‐type and hts1 mutant plants were measured as previously described (Chen et al., 2018 (link)). Cell death was observed using trypan blue staining as previously described (Wu et al., 2015 (link)). H2O2 accumulation was determined by staining with 3,3′‐diaminobenzidine (DAB) (Chen et al., 2010 (link)), or quantified using an Amplex Red hydrogen peroxide/peroxidase assay kit (Invitrogen) following the manufacturer’s instructions. The antioxidant enzyme activities and malondialdehyde (MDA) contents were determined according to Chen et al. (2010 (link)).
Ion leakage was measured as previously described (Shen et al., 2015 ). Briefly, three leaves of 2‐wk‐old rice seedlings harvested after 0, 12, 24 and 48 h of heat stress were trimmed into small pieces and placed in glass tubes containing 10 ml deionized water and incubated overnight at 25°C with shaking (c. 100 rpm). Initial ion leakage (I0) was determined with a conductivity meter (Mettler Toledo, OH, USA). Total ion leakage (It) was measured after 15 min of boiling. Relative ion leakage was expressed as a percentage (I0/It).
Ion leakage was measured as previously described (Shen et al., 2015 ). Briefly, three leaves of 2‐wk‐old rice seedlings harvested after 0, 12, 24 and 48 h of heat stress were trimmed into small pieces and placed in glass tubes containing 10 ml deionized water and incubated overnight at 25°C with shaking (c. 100 rpm). Initial ion leakage (I0) was determined with a conductivity meter (Mettler Toledo, OH, USA). Total ion leakage (It) was measured after 15 min of boiling. Relative ion leakage was expressed as a percentage (I0/It).
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