Porcine Macrophage Generation from Blood

Five cross-bred pigs (Sus scrofa domesticus), either male or female, 6–18 months old, were used to donate blood for macrophage generation. Animals were kept at the Experimental Station of Istituto Zooprofilattico Sperimentale (IZS) of Sardinia (Sassari, Italy). Animal husbandry, handling, and procedures (bleeding) were performed in accordance to the Italian Legislative Decree n.26 dated 4th of March 2014, as well as the Guide of Use of Laboratory Animals issued by the Italian Ministry of Health (authorization n° 1232/2020-PR). Animal health status was controlled by authorized veterinarians, and samples (EDTA blood) were routinely screened for main porcine pathogens, as previously described (22 (link)). In detail, qualitative real-time PCR was employed to exclude the presence of porcine parvovirus (PPV), porcine circovirus 2 (PCV2), and African swine fever (ASFV) genome (22 (link), 23 (link)), with primers reported in the Table S1 (24 (link)–26 (link)), whereas commercial real-time PCR kits were used to detect porcine reproductive and respiratory syndrome virus (PRRSV), and Mycoplasma hyopneumoniae genome (LSI VetMAX™ PRRSV EU/NA and VetMAX™-Plus qPCR Master Mix, both Thermo Fisher Scientific, respectively) (22 (link)).

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Franzoni G., Mecocci S., De Ciucis C.G., Mura L., Dell’Anno F., Zinellu S., Fruscione F., De Paolis L., Carta T., Anfossi A.G., Dei Guidici S., Chiaradia E., Pascucci L., Oggiano A., Cappelli K, & Razzuoli E. (2023). Goat milk extracellular vesicles: immuno-modulation effects on porcine monocyte-derived macrophages in vitro. Frontiers in Immunology, 14, 1209898.

Publication 2023

LSI VetMAX™ PRRSV EU/NA VetMAX™-Plus qPCR Master Mix

African swine fever Animal Blood Edta Female Genome Laboratory animals Macrophage Male Mycoplasma hyopneumoniae Pathogens Porcine Porcine circovirus Porcine parvovirus Porcine reproductive and respiratory syndrome virus Primers Real time pcr Sus scrofa Veterinarians

Corresponding Organization : University of Perugia

Other organizations : Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta, University of Sassari

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Variable analysis

independent variables

Animal sex (male or female)
Animal age (6–18 months)

dependent variables

Macrophage generation from donated blood

control variables

Animal species (Sus scrofa domesticus)
Number of animals (five cross-bred pigs)
Animal health status (controlled by authorized veterinarians and screened for porcine pathogens)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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