Inhibiting 15-PGDH Activity in Mice

CAY10397, a selective inhibitor of 15-Pgdh, was synthesized as previously described and dissolved in 3.3% v/v cell culture grade DMSO and 0.1M sodium carbonate following dilution with PBS (17 (link)). One day prior to infection, C57BL/6 mice on Se-A diet were administered 75 mg/kg of CAY10397 via oral gavage, a concentration that was effective in inhibiting 15-Pgdh activity in tissues by ~65% (17 (link)). These mice were gavaged every alternate day until day 21 PI. To examine the role of 15d-PGJ₂ in Se-D mice, 15d-PGJ₂ (Cayman Chemicals) was used. 15d-PGJ₂ was evaporated under a stream of nitrogen and dissolved in PBS. One day prior to infection, C57BL/6 mice on Se-D diet were treated with 0.05 mg/kg of 15d-PGJ₂ via intraperitoneal injection. The mice were treated daily until they cleared the infection.