Genotyping of Obesity-associated SNPs

5mL of peripheral venous blood were collected from each subject. Then, the genomic DNA was extracted using the standard phenol-chloroform method for genotyping. SNPs were selected based on positive reported variants for obesity from previous GWAS studies.13 (link)–15 (link),18 (link) Six SNPs (rs6499640, rs1421085, rs8050136, rs3751812, rs9939609 and rs9930506) were genotyped in this study. Among them, all these six SNPs were successfully genotyped by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry using the MassARRAY^® Analyzer 4 platform (Sequenom, San Diego, CA). The probes and primers were designed using Mysequenom online software with the accompanying Assay Design Suite v2.0. The standard polymerase chain reaction (PCR) was performed in a total volume of 5 μL containing 10 ng of genomic DNA. Detailed information regarding the primers and PCR reaction conditions is available on request.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Gu Z., Bi Y., Yuan F., Wang R., Li D., Wang J., Hu X., He G., Zhang L, & Liu B.C. (2020). FTO Polymorphisms are Associated with Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) Susceptibility in the Older Chinese Han Population. Clinical Interventions in Aging, 15, 1333-1341.

Publication 2020

MassARRAY® Analyzer 4 platform

Assay Chloroform Genomic Genotyping method Gwas studies Matrix assisted laser desorption ionization mass spectrometry Obesity Phenol Polymerase chain reaction Primers Snps Venous blood

Corresponding Organization :

Other organizations : Shanghai University of Traditional Chinese Medicine, Shanghai Jiao Tong University

Top 5 similar protocols

Variable analysis

independent variables

Rs6499640
Rs1421085
Rs8050136
Rs3751812
Rs9939609
Rs9930506

dependent variables

Obesity

control variables

Genomic DNA extraction using the standard phenol-chloroform method
Genotyping using matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry on the MassARRAY® Analyzer 4 platform
PCR reaction volume of 5 μL with 10 ng of genomic DNA

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!