Culturing Human and Mouse Endothelial Cells

Human Umbilical Vein endothelial cells (HUVECs) were purchased and cultured in an all-in-one complete endothelial growth medium that contains 5 mM Glucose (Cat No.: 211-500) from Cell Applications Inc. (San Diego, CA, USA). HUVECs were purchased from Cell Applications and cultured in HUVEC growth medium [8 (link)]. C57BL/6 Mouse Primary Skeletal Muscle Microvascular Endothelial Cells (Cat No.: C57-6220) were purchased and cultured in Complete Mouse Endothelial Cell Medium (Cat: M1168) from Cell Biologics (Chicago, IL, USA).

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Kuppuswamy S., Annex B.H, & Ganta V.C. (2022). Targeting Anti-Angiogenic VEGF165b–VEGFR1 Signaling Promotes Nitric Oxide Independent Therapeutic Angiogenesis in Preclinical Peripheral Artery Disease Models. Cells, 11(17), 2676.

Publication 2022

HUVECs complete endothelial growth medium Complete Mouse Endothelial Cell Medium

Biologics C57bl mouse Cell Cultured medium Endothelial Endothelial cell Glucose Human umbilical vein endothelial cells Mouse Skeletal muscle cells

Corresponding Organization : Augusta University Health

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Variable analysis

independent variables

Glucose concentration (5 mM)

dependent variables

Cell growth and proliferation of HUVECs
Cell growth and proliferation of C57BL/6 Mouse Primary Skeletal Muscle Microvascular Endothelial Cells

control variables

Cell culture conditions (temperature, CO2 levels, etc.)
Cell culture media composition (except for glucose concentration)

controls

Positive control: Not specified
Negative control: Not specified

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