MDA-MB-231 (HTB-26), HCT-116(CCL-247), LnCaP (Clone FGC, CRL-1740), RH30 (CRL-2061), SJSA (CRL-2098), HOS (CRL-1543), SAOS2 (HTB-85) and U2-OS (HTB-96) cells were purchased from ATCC (Manassas, VA). MCF-7 (86012803) was purchased from Sigma Aldrich (St. Louis, MO). CF-1 is a cell culture isolated from an 18 month old male presenting with alveolar rhabdomyosarcoma (22 ). PCB-204 is a cell culture isolated from the high-grade, extra-skeletal osteosarcoma of a 33 year old male (23 ). MCF-7 and MDA-MB-231 were cultured in DMEM media (Thermo Fisher, MA, 11995073) supplemented with 10% fetal bovine serum (Thermo Fisher, 10437036) and 1% penicillin/ streptomycin (Thermo Fisher, 15140112). All other human cells were cultured in complete RPMI media (Thermo Fisher, 11875119) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. 61323 is an osteosarcoma cell culture isolated from a tumor arising from an Osx1-Cre, Trp53−/−, Rb1−/− genetically engineered mouse as previously characterized (24 (link)). 61323 was cultured in DMEM media supplemented with 10% fetal bovine serum and 1% penicillin/ streptomycin. All human cell cultures were authenticated using short tandem repeat analysis performed by the University of Arizona Genetics Core (Tuscon, AZ).