Regular ob‐DCC media was removed from each well 48 h prior to the 14‐ or 21‐day timepoint and replaced with FBS‐free DCC media. At 14 or 21 days, FBS‐free DCC media was collected from each well and stored at −80°C. A colorimetric dimethylmethylene blue assay was used to quantify sulfated glycosaminoglycan (sGAG) content in the media using chondroitin sulfate as the standard curve as described previously.
28 (link)