Sample processing and flow cytometric analysis of mouse blood cells were performed as described elsewhere [23 (link)]. Briefly, an ethylenediaminetetraacetic acid–coated tube was used to collect mouse peripheral blood. Red blood cell lysis was performed by adding eBioscience 1X RBC Lysis Buffer (Invitrogen). The cells were blocked with Mouse BD FcBlock (BD Biosciences) and labeled with antibody cocktail (Supplementary Table 2 ). Sample acquisition was performed using BD FACSLyric flow cytometry (BD Biosciences), and data were analyzed using FlowJo software (version 10.8; Tree Star).
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