HS-5 cells (ATCC) were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (both from Thermo Fisher Scientific) at 37 °C. The medium was refreshed every two days, and the cells were passaged every 3–4 days using trypsin–EDTA [23 (link)].
HUAECs were cultured in EGM-2 medium (Lonza, Basel, Switzerland) at 37 °C [16 (link)]. The medium was refreshed every two days, and the cells were passaged every 3–4 days using TrypLE Select.
hFOB 1.19 cell line (Cell Bank/Stem Cell Bank of the Chinese Academy of Sciences) was cultured in DMEM/F12 medium supplemented with 10% FBS and 0.3 mg/ml G418 (Thermo Fisher Scientific) at 33.5°. The medium was refreshed every two days, and the cells were passaged every 4–5 days using TrypLE Select [14 (link)].
HUAECs were cultured in EGM-2 medium (Lonza, Basel, Switzerland) at 37 °C [16 (link)]. The medium was refreshed every two days, and the cells were passaged every 3–4 days using TrypLE Select.
hFOB 1.19 cell line (Cell Bank/Stem Cell Bank of the Chinese Academy of Sciences) was cultured in DMEM/F12 medium supplemented with 10% FBS and 0.3 mg/ml G418 (Thermo Fisher Scientific) at 33.5°. The medium was refreshed every two days, and the cells were passaged every 4–5 days using TrypLE Select [14 (link)].
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