Analytical Characterization of Purified Taxol

The purified taxol substance by HPLC from the above steps was subjected to instrumental analysis [48 (link),49 (link),50 (link),51 (link),52 (link)]. Fourier transform infrared (FTIR) and ultraviolet (UV) spectra of the suspected taxol molecule were carried out as described previously in the microanalytical center, Cairo University (Egypt) [13 (link),53 (link)]. ¹H and ¹³C NMR spectra were carried out (JEOL, ECA-500II, 500MHz NMR, Faculty of Science, Mansoura University, Mansoura, Egypt) in comparison to authentic taxol. Samples were dissolved in Deut-rated chloroform (CDCl₃), chemical shifts were given in ppm (δ-scale) and coupling constants were expressed in hertz (Hz) [52 (link),54 ].

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El-Sayed A., Enan G., Al-Mohammadi A.R., H. Moustafa A, & El-Gazzar N. (2020). Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by Penicillium chrysogenum. Molecules, 25(20), 4822.

Publication 2020

ECA-500II

13c nmr Chloroform Faculty Hplc Taxol

Corresponding Organization : Zagazig University

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Variable analysis

independent variables

Purified taxol substance by HPLC

dependent variables

Fourier transform infrared (FTIR) and ultraviolet (UV) spectra of the suspected taxol molecule
¹H and ¹³C NMR spectra

control variables

Authentic taxol for comparison in NMR spectra

controls

Authentic taxol as a positive control for NMR spectra

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