Kidney Function Evaluation Protocol

To evaluate kidney function, we measured serum blood urea nitrogen (BUN) and creatinine (Cre) with commercial kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer's protocols. The commercial kit for measurement of Cre was based on creatinine acid oxidase method as previous reports [40 (link),41 (link)]. The levels of urine Fg were measured using commercially available species-specific Luminex-based assay kit from Millipore (Billerica, MA, USA). The levels of serum Cystatin C were analyzed by a mouse Cystatin C commercial kit (Elabscience Biotechnology Co.,Ltd, Wuhan, China). Subsequently, the optical density for measurement of serum Cystatin C was measured using a microplate reader (SYNERGY H4, BioTek, VT, USA) at the absorbance of 450 nm.

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Lu Q.B., Du Q., Wang H.P., Tang Z.H., Wang Y.B, & Sun H.J. (2019). Salusin-β mediates tubular cell apoptosis in acute kidney injury: Involvement of the PKC/ROS signaling pathway. Redox Biology, 30, 101411.

Publication 2019

Luminex-based assay kit SYNERGY H4

Acid Assay Blood urea nitrogen Creatinine Cystatin c Kidney Mouse cystatin c Optical Oxidase Serum

Corresponding Organization : National University of Singapore

Other organizations : Zhongda Hospital Southeast University

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Variable analysis

dependent variables

Serum blood urea nitrogen (BUN)
Serum creatinine (Cre)
Urine Fg levels
Serum Cystatin C levels

controls

Positive control: None mentioned
Negative control: None mentioned

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