LN-ODAF were prepared by a solvent-diffusion technique [22 (link)], using ODAF as the lipid phase and Lutrol F68® (Poloxamer 188) as the surfactant.
ODAF (0.0115 g) was solubilized in ethanol (2.3 mL) and maintained in a fluid state at 50 °C. The aqueous phase was constituted by hydroxypropylmethyl cellulose (0.115 g), soy lecithin (0.115 g), Lutrol F68® (0.115 g) and distilled water (11.5 mL). Lipid melted phase was slowly dispersed into the hot aqueous surfactant solution (50 °C), by using a high-speed stirrer (Ultra-Turrax T25, IKA-Werke GmbH &Co. Kg, Staufen, Germany) at 15,000 rpm for eight minutes. The obtained coarse emulsion was ultrasonified by using an ultrasonic processor (UP 400 S, Dr. Hielscher GmbH, Stuttgart, Germany) for ten minutes. Then the hot nanoemulsion was dipped in an ice bath for five minutes to obtain LN-ODAF. Finally, the organic solvent was removed by vacuum.
ODAF (0.0115 g) was solubilized in ethanol (2.3 mL) and maintained in a fluid state at 50 °C. The aqueous phase was constituted by hydroxypropylmethyl cellulose (0.115 g), soy lecithin (0.115 g), Lutrol F68® (0.115 g) and distilled water (11.5 mL). Lipid melted phase was slowly dispersed into the hot aqueous surfactant solution (50 °C), by using a high-speed stirrer (Ultra-Turrax T25, IKA-Werke GmbH &Co. Kg, Staufen, Germany) at 15,000 rpm for eight minutes. The obtained coarse emulsion was ultrasonified by using an ultrasonic processor (UP 400 S, Dr. Hielscher GmbH, Stuttgart, Germany) for ten minutes. Then the hot nanoemulsion was dipped in an ice bath for five minutes to obtain LN-ODAF. Finally, the organic solvent was removed by vacuum.
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