Serum circulating cell-free mitochondrial DNA (ccf-mtDNA) was quantified by first isolating DNA from 100 µl of serum using Qiagen DNeasy blood DNA extraction kit (69504), according to manufacturer’s instructions. Duplexed Taqman (iTaq, Bio-Rad) amplification of the mitochondrially-encoded genes MT-ND1 and MT-ND4 is then undertaken using targeted probes as previously described.37 ,38 (link)
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