RNA was extracted from cells using TRIreagent (Sigma) following manufacturer’s instructions and RNA integrity assessed using a Bioanalyzer 6000 pico chip (Agilent). mRNA-seq libraries were prepared using the NEBNext Ultra (or Ultra II) Directional RNA Kit (NEB) with the NEBNext Poly(A) mRNA Magnetic Isolation Module and processed for sequencing as previously described in (39 (link)).
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