Cell Cycle Analysis of rAF-IL12-Treated HT29 Cells

The rAF-IL12-treated HT29 cells (72-h post-infection) was subjected to cell cycle analysis using the Cycletest^™ Plus DNA Reagent Kit (BD Biosciences, Franklin Lakes, NJ, USA). The cells pellet was first resuspended in solution A (trypsin), followed by in solution B (RNAse A) and finally stained with PI dyes, which requires a 10 mins of incubation period in each step. Later, the mixture was analysed by NovoCyte Flow Cytometer (ACEA Biosciences Inc., San Diego, CA, USA) using NovoExpress^® version 1.2.4 software (ACEA Biosciences Inc., San Diego, CA, USA) (Syed Najmuddin et al., 2016 (link)).