The Pseudomonas aeruginosa PAO1 (ATCC 15692) reference strain was used. Bacteria were stored at −70 °C in Trypticase Soy Broth (TSB, Becton Dickinson and Company, Cockeysville, MD, USA) supplemented with 20% glycerol. Pseudomonas phages KT28, KTN4, and LUZ19 were propagated as previously described [62 (link)]. The phage titre was assessed using the double-agar layer technique. Phage culture purity was also confirmed genetically using specific primers, listed in Table S3, to exclude potential contamination. Purified phage samples were stored at 4 °C. Phage characteristics are presented in Table 3.
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