Transient and Heritable Zebrafish Genetic Manipulations
Corresponding Organization :
Other organizations : Chinese Academy of Sciences, Shanghai Institute of Nutrition and Health, Qingdao Agricultural University, Ruijin Hospital, Shanghai Jiao Tong University, Shanghai Institute of Hematology, Boston Children's Hospital, Harvard University, Baotou Medical College
Variable analysis
- Morpholino oligonucleotides (MOs)
- Transient tprb transgene construct within Tol2 vectors (40 pg)
- CRISPR-Cas9 gRNAs (50 pg) and Cas9 protein (500 pg)
- Phenotype/effects of MO knockdown
- Phenotype/effects of tprb transgene expression
- Phenotype/effects of tprb and vhl gene mutations
- Microinjection of one-cell-stage embryos
- Tol2 transposase mRNA (50 pg) co-injected with tprb transgene construct
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!