PBMCs were stimulated for as above in the presence of 2.5µg/ml anti-CD107α, 1.25µg/ml anti-CD107b (BD Bioscience) and 10µM TAPI-2 (VWR International, West Sussex, UK). Following stimulation, cells were resuspended in cytometry buffer (PBS + 0.05% sodium azide + 2mM EDTA + 2% fetal calf serum) and stained with isotope-tagged antibodies before being acquisition on the CyTOF as previously described19 (link).
Protocol detail
CyTOF Profiling of Stimulated PBMCs
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : University of Oxford, John Radcliffe Hospital, Medawar Building for Pathogen Research, Newcastle University, National University of Singapore, MRC Weatherall Institute of Molecular Medicine, Stanford University, Singapore Immunology Network, Agency for Science, Technology and Research, Stockholm University, University of Otago
Top 5 similar protocols
Variable analysis
independent variables
- Stimulation with anti-CD107α (2.5µg/ml)
- Stimulation with anti-CD107b (1.25µg/ml)
- Treatment with TAPI-2 (10µM)
- Acquisition of cells on CyTOF
- Cytometry buffer (PBS + 0.05% sodium azide + 2mM EDTA + 2% fetal calf serum)
- Staining with isotope-tagged antibodies
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!