Lung Tissue Protein Expression Analysis

The lung tissue was homogenized (1/10 w/v) using a homogenizer in tissue lysis/extraction reagent (Sigma-Aldrich) supplemented with protease inhibitors (Sigma-Aldrich). Immunoblotting was performed as described previously [12 (link)]. The following primary antibodies were used: anti-PDE4B (1:1000 dilution; Abcam), anti-MMP-9 (1:1000 dilution; Abcam), and anti-β-actin (1:1000 dilution; Cell signaling, Denver, MA, USA). Expression of PDE4B, MMP-9, and β-actin were evaluated using ChemiDoc (Bio-Rad, Hercules, CA, USA).

Free full text: Click here

Lim J.O., Kim W.I., Lee S.J., Pak S.W., Cho Y.K., Kim J.C., Kim J.S, & Shin I.S. (2021). The Involvement of PDE4 in the Protective Effects of Melatonin on Cigarette-Smoke-Induced Chronic Obstructive Pulmonary Disease. Molecules, 26(21), 6588.

Publication 2021

tissue lysis/extraction reagent protease inhibitors anti-PDE4B anti-MMP-9 anti-β-actin ChemiDoc

Actin Antibodies Dilution Lung Mmp 9 Pde4b Protease inhibitors Tissue

Corresponding Organization :

Other organizations : Chonnam National University, Cheongju University

Top 5 similar protocols

Variable analysis

independent variables

Homogenization of lung tissue using a homogenizer in tissue lysis/extraction reagent (Sigma-Aldrich) supplemented with protease inhibitors (Sigma-Aldrich)

dependent variables

Expression of PDE4B
Expression of MMP-9
Expression of β-actin

control variables

Dilution of primary antibodies used for immunoblotting (1:1000 for anti-PDE4B, anti-MMP-9, and anti-β-actin)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!