NPS were collected by a health care professional and were placed directly onto a viral transport medium before transport to the laboratory. Fresh, self-collected saliva were transported to the laboratory without any viral transport media and were then diluted in phosphate-buffered saline (PBS) and lysis buffer before amplification (5 (link)).
Molecular detection was based on the RT-PCR amplification of at least two viral genome regions. The assays used by our laboratory during the study period were the Cobas SARS-CoV-2 assay (“ROC”, Roche Molecular Diagnostics, Mannheim, Germany), Alinity m SARS-CoV-2 assay (“ALI”, Abbott Molecular, Rungis, France), NeuMoDx SARS-CoV-2 assay (“NMDx”, Qiagen, Courtaboeuf, France), Xpert Xpress SARS-CoV-2 assay (“CPH”, Cepheid, Maurens-Scopont, France), and Simplexa COVID-19 Direct assay (“SPX”, DiaSorin, Antony, France).
Our laboratory information system collected results expressed as cycle threshold (Ct) values. For assays with multiple targets, the mean Ct value was calculated.
Molecular detection was based on the RT-PCR amplification of at least two viral genome regions. The assays used by our laboratory during the study period were the Cobas SARS-CoV-2 assay (“ROC”, Roche Molecular Diagnostics, Mannheim, Germany), Alinity m SARS-CoV-2 assay (“ALI”, Abbott Molecular, Rungis, France), NeuMoDx SARS-CoV-2 assay (“NMDx”, Qiagen, Courtaboeuf, France), Xpert Xpress SARS-CoV-2 assay (“CPH”, Cepheid, Maurens-Scopont, France), and Simplexa COVID-19 Direct assay (“SPX”, DiaSorin, Antony, France).
Our laboratory information system collected results expressed as cycle threshold (Ct) values. For assays with multiple targets, the mean Ct value was calculated.
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