Tumor mouse models were created by orthotopic pancreatic injections of 5 × 105 LM-PmC cells into nude mice purchased from Envigo (Indianapolis, IN) and 105 KPC-derived PDAC organoids into C57BL6/129 F1 hybrid mice (Jackson, Bar Harbor, ME). In some cases, luciferase-labeled KPC-derived PDAC organoids were used, and their growth was monitored by luminescence imaging of the mice using a Xenogen IVIS Spectrum Imaging System (PerkinElmer, Waltham, MA). The p48-CRE, LSL-KrasG12D, INK4aflox mice required for the experiments were kindly provided by Dr. Douglas Hanahan (current affiliation: Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland). KPC mice were maintained as previously described52 (link).
The mice were maintained at a maximum of 5 mice per cage with 12 h-light/dark cycles, 20 °C, and 50% humidity. All animal experiments were performed according to procedures approved by the Animal Research Committees at Sanford–Burnham–Prebys (SBP) Medical Discovery Institute (La Jolla, CA) and University of California San Diego (UCSD, La Jolla, CA).
The mice were maintained at a maximum of 5 mice per cage with 12 h-light/dark cycles, 20 °C, and 50% humidity. All animal experiments were performed according to procedures approved by the Animal Research Committees at Sanford–Burnham–Prebys (SBP) Medical Discovery Institute (La Jolla, CA) and University of California San Diego (UCSD, La Jolla, CA).
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