Monitoring Olvi-vec Viral Presence

Viral plaque assays were performed on body fluid samples (blood, sputum, urine, pleural fluid) collected from patients immediately before and 24, 48, 72, and 96 hours after Olvi-vec treatment to assess for the presence of viral particles. Post-treatment tumor biopsies collected 2-5 days after treatment also underwent assessment for viral particles using viral plaque assays. In brief, patient samples were plated on confluent layers of CV-1 cells. Evaluation of virus infection was done by visual assessment of viral plaque in wells with both CV-1 cells and patient samples. Additionally, post-treatment serum samples obtained from patients 60 days after Olvi-vec treatment were assessed for the presence of Olvi-vec neutralizing antibodies via standard vaccinia virus neutralization assay and compared to corresponding pre-treatment serum samples.

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Chintala N.K., Choe J.K., McGee E., Bellis R., Saini J.K., Banerjee S., Moreira A.L., Zauderer M.G., Adusumilli P.S, & Rusch V.W. (2023). Correlative analysis from a phase I clinical trial of intrapleural administration of oncolytic vaccinia virus (Olvi-vec) in patients with malignant pleural mesothelioma. Frontiers in Immunology, 14, 1112960.

Publication 2023

After treatment Assay Biopsies Blood Body fluid Cells Neutralizing antibodies Patients Plaque Pleural Samples treatment Serum Sputum Tumor Urine Vaccinia virus Viral particles Viral plaque assays Virus infection

Corresponding Organization : Memorial Sloan Kettering Cancer Center

Other organizations : New York University

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Variable analysis

independent variables

Olvi-vec treatment

dependent variables

Presence of viral particles in body fluid samples (blood, sputum, urine, pleural fluid) before and after Olvi-vec treatment
Presence of viral particles in post-treatment tumor biopsies
Presence of Olvi-vec neutralizing antibodies in patient serum samples 60 days after treatment

control variables

Confluent layers of CV-1 cells used to assess viral infection
Pre-treatment serum samples used as control for Olvi-vec neutralizing antibody assay

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