BN rats were anesthetised on Day 53 by injection of hypnorm-dormicum and initial ear thickness was measured twice. Subsequently, an EST was performed by intradermally injecting 10 µg of PPE in 20 µL PBS into right ear of each rat as previously described (34 (link)). Ear thickness was measured again 30 min after the injection and ear swelling was determined as a measure of the clinical relevance of the peanut sensitisation.
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