Wheat gluten obtained from ‘Bainong 207,’ Xinxiang Nongle Seed Industry Co., Ltd. (Xinxiang, China). Bacillus subtilis B53 is a laboratory preservation strain (Henan Province Industrial microbial species Preservation Center). SDS-PAGE kit was purchased from Solarbio (Wuhan, China). BCA protein assay kit was obtained from Glpbio (lot no. GK10009, Shanghai, China). All aqueous solutions were prepared with ultrapure water. All the chemicals used in this study were of analytical grade.
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