Cell Cycle Analysis by Flow Cytometry

TIVE or TIVE-LTC cells were either untreated or treated with drugs (Indo or NS-398) or solvent control as described for cell number and viability assays. Harvested cells were diluted to contain ∼10⁶ cells/ml and DNA distribution analysis was performed. Cells were fixed with 70% ethanol overnight and DNA was stained with propidium iodide at a final concentration of 50 µg/ml with RNaseA (100 U/ml) prior to flow cytometry analysis using a LSRII (BD Biosciences). Data were analyzed using ModFit Lt V3 software (Verity Software House).

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Sharma-Walia N., Paul A.G., Bottero V., Sadagopan S., Veettil M.V., Kerur N, & Chandran B. (2010). Kaposi's Sarcoma Associated Herpes Virus (KSHV) Induced COX-2: A Key Factor in Latency, Inflammation, Angiogenesis, Cell Survival and Invasion. PLoS Pathogens, 6(2), e1000777.

Publication 2010

Assays Cells Drugs Ethanol Flow cytometry Ns 398 Propidium iodide Solvent

Corresponding Organization :

Other organizations : Rosalind Franklin University of Medicine and Science

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Protocol cited in 5 other protocols

Variable analysis

independent variables

Treatment with drugs (Indo or NS-398)
Solvent control

dependent variables

DNA distribution analysis
Cell number and viability

control variables

Cell density (∼10^6 cells/ml)
DNA staining with propidium iodide (50 µg/ml) and RNaseA (100 U/ml)
Flow cytometry analysis using a LSRII (BD Biosciences)
Data analysis using ModFit Lt V3 software (Verity Software House)

controls

Untreated cells (negative control)

Annotations

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