Glioblastoma Cell Line Characterization

Human U87-MG glioblastoma cells (ATCC HTB-14) were modified by lentiviral infection to stably express fLuc2 and eGFP (U87-fLuc2/eGFP, maximum excitation λ = 488 nm, maximum emission λ = 509 nm) under the control of the CMV promoter (kindly provided by Dr. S.A. Collins from Department of Medicine-Digestive Diseases, UCLA, USA)48 (link). They were grown at 37 °C in a 5% CO₂ humidified atmosphere, in Modified Essential Medium EARLES (10370–047, Gibco, France) supplemented with 10% fetal bovine serum (10500–064, Gibco, France), 0.2% Glucose (19002–013, Gibco, France), 2 mM L-glutamine (X0550, Dominique Dutscher, France) and 100 U/ml penicillin and 100 μg/ml streptomycin (15140155, Gibco, France). The viability of U87 cells was greater than 90% as determined by visual counts using Trypan Blue Dye Exclusion on a Malassez cell, or by analysis with the Muse^® Cell Analyzer (MCH100102, Muse Count & Viability Assay Kit, Millipore).