IM-0223 cells (2 × 104 cells) were incubated in 8-well chamber slides (Thermo Fisher Scientific), and stained for immunofluorescence as previously described (28 (link)), using the following Abs: mouse anti-human KPC1 Ab (1:50 dilution, #ab57549; Abcam), rabbit anti-human NF-κB1 p50 Ab (1:50 dilution, #sc-114; Santa Cruz Biotechnology), Alexa Fluor 488-conjugated goat anti-mouse IgG (2.5 μg/ml, #115-545-003, Jackson ImmunoResearch, West Grove, PA) or Cy3-conjugated goat anti-rabbit IgG (2.5 μg/ml, #111-165-003, Jackson ImmunoResearch). The slides were stained with 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) in mounting medium. Images were obtained using a Nikon Eclipse Ti microscope and NIS elements software (Nikon).
Protocol detail
Immunofluorescence Analysis of KPC1 and NF-κB1 p50
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Molecular Oncology (United States)
Other organizations : Technion – Israel Institute of Technology, The University of Texas MD Anderson Cancer Center
Top 5 similar protocols
Variable analysis
independent variables
- Anti-human KPC1 antibody (mouse) at 1:50 dilution
- Anti-human NF-κB1 p50 antibody (rabbit) at 1:50 dilution
- Alexa Fluor 488-conjugated goat anti-mouse IgG at 2.5 μg/ml
- Cy3-conjugated goat anti-rabbit IgG at 2.5 μg/ml
- Fluorescence signals of KPC1 and NF-κB1 p50 proteins in IM-0223 cells
- IM-0223 cells (2 × 10^4 cells)
- 8-well chamber slides (Thermo Fisher Scientific)
- DAPI staining in mounting medium
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!