At 48 or 72 h after transfection, the cell extracts were prepared and immunoblot assays performed as described elsewhere [25 (link)–27 (link)]. The following antibodies were used: PKR (B-10), PKR (K-17) and beta-actin (Santa Cruz Biotechnology, Santa Cruz, CA); phospho-PKR [pT451] and phospho-eIF-2α [pS51] (BioSource International, Camarillo, CA).
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