Human gastric cancer cell SGC7901 was provided by the Shanghai Cell Collection (Shanghai, China). Cells were cultured in RPMI-1640 medium (Gibco Laboratories, USA) containing 10% (v/v) fetal bovine serum, 1% penicillin–streptomycin 100 U/ml penicillin, and 100 μg/ml streptomycin in a humidified atmosphere of 5% CO2 in air at 37°C.
DDP-resistant SGC7901/DDP cells were induced from SGC7901 cells, using a concentration gradient method to increase the half maximal inhibitory concentration (IC50) of DDP (as previously described) [7 (link)].
DDP-resistant SGC7901/DDP cells were induced from SGC7901 cells, using a concentration gradient method to increase the half maximal inhibitory concentration (IC50) of DDP (as previously described) [7 (link)].
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