Acquisition and Culture of Burkholderia Strains

B. pseudomallei Bp82 (Propst et al., 2010 (link)), a ΔpurM mutant that is avirulent in immunocompetent and immunodeficient animals and is exempt from select-agent rules, was obtained from H. Schweizer. B. cenocepacia BC7, K56-2S, K56-2V (Varga et al., 2013 (link)) were obtained from J. Goldberg. B. multivorans CGD1 and CGD2 (Varga et al., 2012 (link)) were obtained from S. Holland. B. thailandensis E264 (Yu et al., 2006 (link)) was obtained from D. DeShazer. For Burkholderia, routine medium was Miller LB (Atlas, 2010 ) broth and agar. Pseudomonas aeruginosa PA14 (He et al., 2004 (link)) liquid medium was Miller LB (Atlas, 2010 ). Ciprofloxacin was obtained from MP Biomedicals (Aurora, Ohio, United States). Levofloxacin, Trimethoprim, Doxycycline, Gentamicin, and Cefotaxime were obtained from Sigma (St. Louis, MO). All bacteria were grown at 37°C in ambient air.