Protocol detail

DLBCL Cell Line Culture and Drug Treatment

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The ABC DLBCL lines TMD8, HBL1, OYB, U2932, SUDHL2, and TK, and the GCB-DLBCL lines BJAB, OCI-Ly1, and OCI-Ly8 were grown in RPMI 1640 plus 20% FBS plus Pen/Strep. ABC DLBCL lines OCI-Ly10, OCI-Ly3 and DLBCL2 were grown in RPMI 1640 plus 20% heparinized human plasma plus Pen/Strep. Cell lines were previously engineered to express an ecotropic retroviral receptor and a bacterial tetracycline repressor [4 (link)]. All drugs (PRT062607, Dasatinib, BYL719, CAL-101, IPI-145, TGX221, CZC24832) were obtained from Selleckchem and diluted in DMSO.

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Pongas G.N., Annunziata C.M, & Staudt L.M. (2017). PI3Kδ inhibition causes feedback activation of PI3Kα in the ABC subtype of diffuse large B-cell lymphoma. Oncotarget, 8(47), 81794-81802.

Publication 2017

PRT062607 Dasatinib BYL719 CAL-101 IPI-145 TGX221 CZC24832

Bacterial Byl719 Cal 101 Cell lines Dasatinib Dmso Drugs Human Ipi 145 Plasma Prt062607 Retroviral Strep Tetracycline

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Variable analysis

independent variables

PRT062607
Dasatinib
BYL719
CAL-101
IPI-145
TGX221
CZC24832

dependent variables

Cell growth and proliferation

control variables

RPMI 1640 plus 20% FBS plus Pen/Strep medium for ABC DLBCL lines TMD8, HBL1, OYB, U2932, SUDHL2, and TK, and GCB-DLBCL lines BJAB, OCI-Ly1, and OCI-Ly8
RPMI 1640 plus 20% heparinized human plasma plus Pen/Strep medium for ABC DLBCL lines OCI-Ly10, OCI-Ly3 and DLBCL2
Cell lines previously engineered to express an ecotropic retroviral receptor and a bacterial tetracycline repressor

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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