Assessing Plasma Membrane Integrity

The SYTOX Green kit (Life Technologies, Madrid, Spain) was used for the evaluation of the cellular plasmatic membrane damage. This stain binds to the DNA of those cells who have suffered alterations in the permeability of the plasmatic membrane and emits a high green fluorescence. Hence, no green fluorescence can be shown in healthy cells. This assay was performed following manufacturer's instruction and modified by Sifaoui et al. (2018) (link). An EVOS M5000 Cell Imaging System (Life Technologies, Madrid, Spain) was used to obtain the images.

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Arberas-Jiménez I., Cen-Pacheco F., Chao-Pellicer J., Sifaoui I., Rizo-Liendo A., Morales E.Q., Daranas A.H., Díaz-Marrero A.R., Piñero J.E., Fernández J.J, & Lorenzo-Morales J. (2023). Identification and characterization of novel marine oxasqualenoid yucatecone against Naegleria fowleri. International Journal for Parasitology: Drugs and Drug Resistance, 22, 61-71.

Publication 2023

EVOS M5000 Cell Imaging System

Assay Cell Emits Fluorescence Membrane permeability Permeability Plasmatic Plasmatic membrane Stain Sytox green

Corresponding Organization : Consejo Superior de Investigaciones Científicas

Other organizations : Universidad Veracruzana

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Variable analysis

independent variables

Plasmatic membrane damage

dependent variables

Green fluorescence

control variables

Healthy cells (no green fluorescence)

controls

Positive control: Cells with altered plasmatic membrane permeability (high green fluorescence)
Negative control: Healthy cells (no green fluorescence)

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