Microscopic Analysis of Fungal Cultures

Cultures were prepared and maintained as described previously (Jaklitsch 2009 (link)). Microscopic observations were made in tap water, except where noted. Morphological analyses of microscopic characters were carried out as described earlier (Jaklitsch 2009 (link)). Methods of microscopy included stereomicroscopy using Nikon SMZ1500, Olympus SZX10 and Euromex Novex RZ 65.560, light microscopy using Euromex XHR MIC 625, Olympus BX51 and Nomarski differential interference contrast (DIC) using the compound microscopes Nikon Eclipse E600 and Zeiss Axio Imager.A1. Images and data were gathered with Nikon Coolpix 4500, Nikon DS-U2, Nikon D90, Olympus DP72 and Zeiss Axiocam 506 colour digital cameras and measured directly with the microscope, or with Olympus cellSens Dimension, NIS-Elements D v.3.0 and Zeiss ZEN Blue Edition softwares. Amyloidity of asci was assessed using Lugol or Melzer reagent. Measurements are reported as maximum and minimum in parentheses and the range representing the mean plus and minus the standard deviation of a number of measurements given in parentheses.

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Voglmayr H., Friebes G., Gardiennet A, & Jaklitsch W.M. (2017). Barrmaelia and Entosordaria in Barrmaeliaceae (fam. nov., Xylariales) and critical notes on Anthostomella-like genera based on multigene phylogenies. Mycological Progress, 17(1), 155-177.

Publication 2017

Axio Imager.A1 Coolpix 4500 Nikon DS-U2 Axiocam 506 cellSens Dimension NIS-Elements D v.3.0 ZEN Blue Edition

Cameras Characters Compound microscopes E600 Microscope

Corresponding Organization :

Other organizations : University of Vienna, Universalmuseum Joanneum, BOKU University

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Variable analysis

independent variables

Microscopic observations were made in tap water, except where noted.

dependent variables

Morphological analyses of microscopic characters were carried out.

control variables

Cultures were prepared and maintained as described previously (Jaklitsch 2009).
Methods of microscopy included stereomicroscopy, light microscopy, and Nomarski differential interference contrast (DIC) using the compound microscopes.

controls

Amyloidity of asci was assessed using Lugol or Melzer reagent.

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