TcdB was isolated from C. difficile strain VPI10463 and obtained from Enzo Life Sciences (BML-G150-0050; Farmingdale, NY, USA). To prepare a stock solution, TcdB was reconstituted to 200 µg/mL and stored at −80 °C, as indicated in the data sheet, before use in the experiments at a concentration of 0.1 ng/mL, 1 ng/mL, and 10 ng/mL [28 (link),37 (link),50 (link)].
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