Nissl staining was performed with 0.1% cresyl violet solution (Sigma-Aldrich) using a standard protocol to evaluate the peri-impact injury of our TBI model 26 (link), 27 (link). From each perfused brain, six coronal sections between the anterior edge and posterior edge of the TBI impact area were collected and processed for Nissl staining. The peri-impact area was defined as the region in the cortex immediately surrounding the TBI impact area, starting at AP +2.0 mm and ending at AP -3.8 mm posterior from the bregma. Every sixth coronal tissue section was chosen at random to quantify cell survival in the peri-impact area. Brain sections were examined using a light microscope (Keyence). Neuronal survival in the peri-impact area of the brain was quantified using a computer-assisted image analysis system (NIH Image Software, USA) and was expressed as a percentage of the ipsilateral hemisphere compared to the contralateral hemisphere 27 (link).
Protocol detail
Nissl Staining for TBI Perilesional Analysis
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : University of South Florida
Top 5 similar protocols
Variable analysis
independent variables
- Nissl staining with 0.1% cresyl violet solution
- Neuronal survival in the peri-impact area of the brain
- Perfused brain samples
- Six coronal sections between the anterior edge and posterior edge of the TBI impact area
- Peri-impact area defined as the region in the cortex immediately surrounding the TBI impact area, starting at AP +2.0 mm and ending at AP -3.8 mm posterior from the bregma
- Every sixth coronal tissue section chosen at random to quantify cell survival in the peri-impact area
- Brain sections examined using a light microscope (Keyence)
- Neuronal survival in the peri-impact area quantified using a computer-assisted image analysis system (NIH Image Software, USA) and expressed as a percentage of the ipsilateral hemisphere compared to the contralateral hemisphere
- Positive control: Not specified
- Negative control: Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!