Bacterial Culture Conditions and Reagents

The bacterial strains and plasmids used in this study are listed in Table 1. All strains were initially grown at 37 °C in LB medium, then tested in EG medium, which is E medium [35 (link)] containing 0.4% glucose [36 (link)]. The medium pH was adjusted by the addition of NaOH or HCl. Antibiotics were used at the following concentrations: ampicillin, 100 µg/mL; kanamycin, 30 µg/mL; chloramphenicol, 30 μg/mL. Antibiotics, L-arabinose, N,N’-dicyclohexylcarbodiimide (DCCD) and carbonyl cyanide 3-chlorophenylhydrazone (CCCP) were obtained from Sigma-Aldrich (St. Louis, MO, USA).

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Zhang W., Chen X., Sun W., Nie T., Quanquin N, & Sun Y. (2020). Escherichia coli Increases its ATP Concentration in Weakly Acidic Environments Principally through the Glycolytic Pathway. Genes, 11(9), 991.

Publication 2020

L-arabinose N,N’-dicyclohexylcarbodiimide (DCCD) carbonyl cyanide 3-chlorophenylhydrazone (CCCP)

Ampicillin Antibiotics Bacterial Carbonyl cyanide 3 chlorophenylhydrazone Cccp Chloramphenicol Dicyclohexylcarbodiimide Glucose Kanamycin L arabinose Plasmids Strains

Corresponding Organization : Chinese Academy of Sciences

Other organizations : South China Agricultural University, Guangdong University of Technology, University of California, Los Angeles

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Variable analysis

independent variables

Medium pH (adjusted by addition of NaOH or HCl)

dependent variables

Bacterial growth/survival

control variables

Bacterial strains and plasmids (as listed in Table 1)
Growth temperature (37 °C)
Growth medium (LB, then EG medium)
Antibiotic concentrations (ampicillin 100 µg/mL, kanamycin 30 µg/mL, chloramphenicol 30 μg/mL)
Chemicals (L-arabinose, N,N'-dicyclohexylcarbodiimide (DCCD), carbonyl cyanide 3-chlorophenylhydrazone (CCCP))

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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