Aβ42 Oligomer Binding Assay

Aβ42 oligomer preparations were generated from synthetic peptide4 (link). For binding assays, COS-7 cells were transiently transfected with cDNA expression plasmids or isolated hippocampal neurons were cultured from E18 embryos. Bound biotin-Aβ42 was detected using avidin conjugates.

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Laurén J., Gimbel D.A., Nygaard H.B., Gilbert J.W, & Strittmatter S.M. (2009). Cellular Prion Protein Mediates Impairment of Synaptic Plasticity by Amyloid-β Oligomers. Nature, 457(7233), 1128-1132.

Publication 2009

Assays Avidin Biotin Cdna Cos 7 cells Embryos Neurons Plasmids

Corresponding Organization :

Other organizations : Yale University

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Variable analysis

independent variables

Aβ42 oligomer preparations generated from synthetic peptide

dependent variables

Binding of biotin-Aβ42 to COS-7 cells and isolated hippocampal neurons

control variables

COS-7 cells transiently transfected with cDNA expression plasmids
Isolated hippocampal neurons cultured from E18 embryos

controls

Positive control: Not specified
Negative control: Not specified

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