Protocol detail

Plasmid and siRNA Protocols for FOXM1B and Met

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The plasmid pcDNA3.1-FOXM1B (pFOXM1) and control vector plasmid pcDNA3.1 were described previously.^{24 (link)} Small interfering RNAs (siRNAs) targeting Met was synthesized by Dharmacon, and the sequence of siRNA#1 5'-GTGCAGTATCCTCTGACAG-3' and siRNA#2 5’-AAGTGCAGTATCCTCTGACAG-3’ were reported previously.^{25 (link),26 (link)} SiRNA targeting FOXM1 (siFOXM1) was described previously.²⁷

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Cui J., Xia T., Xie D., Gao Y., Jia Z., Wei D., Wang L., Huang S., Quan M, & Xie K. (2016). HGF/Met and FOXM1 Form a Positive Feedback Loop and Render Pancreatic Cancer Cells Resistance to Met Inhibition and Aggressive Phenotypes. Oncogene, 35(36), 4708-4718.

Publication 2015

siRNAs siFOXM1

Plasmid Sirna

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Variable analysis

independent variables

Transfection of pcDNA3.1-FOXM1B (pFOXM1) plasmid
Transfection of siRNA targeting Met (siRNA#1 and siRNA#2)
Transfection of siRNA targeting FOXM1 (siFOXM1)

dependent variables

Outcome variables not explicitly mentioned

control variables

Transfection of control vector plasmid pcDNA3.1

controls

Positive control: Transfection of pcDNA3.1-FOXM1B (pFOXM1) plasmid
Negative control: Transfection of control vector plasmid pcDNA3.1

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