To
evaluate the cellular uptake and mucoadhesive properties of thiolated
CDs, coumarin-6, a lipophilic fluorescent dye, was host–guest
complexed with native β-CD, β-CD-SH, and β-CD-SS-MESNA
following a previously described method.6 Briefly, 1 mL of ethanolic coumarin-6 solution (0.02% m/V) was added
to 50 mL of 100 mM phosphate buffer pH 6.5 containing 50 mg of CD.
The dispersions were stirred in the dark for 24 h at room temperature,
filtered to eliminate the free dye and nondissolved CDs, and lyophilized
for 2 days. The formed complexes were characterized based on their
fluorescence in 100 mM phosphate buffer (pH 6.8) and ethanol, as described
previously.10 (link)
evaluate the cellular uptake and mucoadhesive properties of thiolated
CDs, coumarin-6, a lipophilic fluorescent dye, was host–guest
complexed with native β-CD, β-CD-SH, and β-CD-SS-MESNA
following a previously described method.6 Briefly, 1 mL of ethanolic coumarin-6 solution (0.02% m/V) was added
to 50 mL of 100 mM phosphate buffer pH 6.5 containing 50 mg of CD.
The dispersions were stirred in the dark for 24 h at room temperature,
filtered to eliminate the free dye and nondissolved CDs, and lyophilized
for 2 days. The formed complexes were characterized based on their
fluorescence in 100 mM phosphate buffer (pH 6.8) and ethanol, as described
previously.10 (link)
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