Western Blot Analysis of HLA-I
Variable analysis
- EndoH treatment
- PNGase treatment
- Detection of human HLA-I heavy chain (HC10 hybridoma)
- Detection of V5-tag (MCA1360, cloneSV5-Pk1, BioRad)
- Actin level (A2066, Sigma-Aldritch)
- Sample loading
- Positive control: Murine monoclonal antibodies for human HLA-I heavy chain (HC10 hybridoma) and V5-tag (MCA1360, cloneSV5-Pk1, BioRad)
- Negative control: Rabbit polyclonal antibody specific for actin (A2066, Sigma-Aldritch)
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