HLA Typing of Glioma Patient Samples

Genomic DNA was isolated from patient blood and glioma tissue using the NucleoSpin Tissue Kit (Macherey-Nagel) or the Maxwell® 16 LEV Blood DNA Kit (Promega). The isolated DNA was HLA-typed as described previously.^{24 (link)}

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Lu K.H., Michel J., Kilian M., Aslan K., Qi H., Kehl N., Jung S., Sanghvi K., Lindner K., Zhang X.W., Green E.W., Poschke I., Ratliff M., Bunse T., Sahm F., von Deimling A., Wick W., Platten M, & Bunse L. (2022). T cell receptor dynamic and transcriptional determinants of T cell expansion in glioma-infiltrating T cells. Neuro-Oncology Advances, 4(1), vdac140.

Publication 2022

NucleoSpin Tissue Kit Maxwell® 16 LEV Blood DNA Kit

Blood Genomic Glioma Patient Promega Tissue

Corresponding Organization : German Cancer Research Center

Other organizations : Essen University Hospital, National Center for Tumor Diseases, University of Mannheim, Helmholtz Institute Mainz, Johannes Gutenberg University Mainz

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Variable analysis

independent variables

Isolated DNA

dependent variables

HLA type

control variables

Isolation of DNA from patient blood and glioma tissue using the NucleoSpin Tissue Kit (Macherey-Nagel) or the Maxwell® 16 LEV Blood DNA Kit (Promega)

controls

Positive control: No positive control explicitly mentioned.
Negative control: No negative control explicitly mentioned.

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