Histological Analysis of Hepatic and Adipose Tissue

Hepatic tissue and epididymal fat were preserved in a 10% formalin (Abcam, USA) solution overnight before being embedded in paraffin (Thermo Fisher Scientific, USA). 4 µm slices were prepared and subsequently deparaffinized with the xylene (Sigma, St Louis, USA) and concentration gradient of ethanol and rehydrated using deionized H₂O for two washes of 5 min. Furthermore, we stained with hematoxylin and eosin (H&E, Harris Hematoxylin and Eosin Y; Sigma, St Louis, USA) (21 (link)), the H&E-stained slides were inspected using a Leica Microsystems CMS GmbH (Wetzlar, Germany), and morphological pictures were analyzed using the SIS 3.2 software (Soft-Imaging System).

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Zhong B., Li F.L., Zhao J.Y., Fu Y, & Peng C. (2022). Sporoderm-broken spore powder of Ganoderma lucidum ameliorate obesity and inflammation process in high-fat diet-induced obese mice. Food & Nutrition Research, 66, 10.29219/fnr.v66.8745.

Publication 2022

formalin paraffin xylene SIS 3.2 software

Embedded paraffin Eosin y Epididymal Ethanol Formalin Hematoxylin Tissue Xylene

Corresponding Organization :

Other organizations : Jilin Agricultural Science and Technology University, People 's Hospital of Jilin Province

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Variable analysis

independent variables

Hepatic tissue and epididymal fat preservation in 10% formalin solution

dependent variables

Morphological evaluation of hepatic tissue and epididymal fat

control variables

Tissue fixation duration (overnight)
Paraffin embedding
Thickness of tissue slices (4 µm)
Deparaffinization using xylene and ethanol gradient
Rehydration using deionized water
Hematoxylin and eosin (H&E) staining
Microscopic imaging using Leica Microsystems CMS GmbH
Image analysis using SIS 3.2 software

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